erdman atcc 35801 strain  (ATCC)

Journal: Cell chemical biology

Article Title: Drugging the Folate Pathway in Mycobacterium Tuberculosis : The Role of Multi-Targeting Agents

doi: 10.1016/j.chembiol.2019.02.013

Figure Lengend Snippet: Mycobacterium tuberculosis folate pathway. Canonical pathway enzyme (green): DHPS: dihydropteroate synthase, DHFS: dihydrofolate synthase, DHFR: dihydrofolate reductase, TS: thymidylate synthase, SHMT: serine hydroxyl methyltransferase; Alternate pathway enzymes (orange) FDTS: flavin-dependent thymidylate synthase, Rv2671

Article Snippet: The accession numbers are provided in . table ft1 table-wrap mode="anchored" t5 caption a7 Reagents or Resources Source Identifier Chemicals Beta-nicotinamide adenine dinucleotide phosphate (NADPH) Alfa-Aesar Cat#J60387 Dihydrofolic acid (DHF) Sigma-Aldrich Cat#D7006 Tetrahydrofolic acid (THF) Sigma-Aldrich Cat#T3125 Methotrexate Sigma-Aldrich Cat#A6770 Isoniazid Sigma-Aldrich Cat#I3377 Flavin adenine dinucleotide disodium salt hydrate (FAD) Sigma-Aldrich Cat#F6625 2’-deoxyuridine-5’-monophosphate disodium salt (dUMP) Alfa-Aesar Cat#J64627 Thymidine 5’-monophosphate disodium salt (dTMP) Sigma-Aldrich Cat#T7004 5,10-Methylenetetrahydrofolate (mTHF) Toronto Research Chemicals Cat#F680350 Isopropyl-1-thio-b-D-galacto-pyranoside (IPTG) Thermo Scientific Cat#R0392 para -Aminosalicylic acid Sigma-Aldrich Cat#A79604 Nextera XT DNA library preparation kit Illumina Cat#FC-131 AMPure XP beads Beckman-Coulter Cat#A63880 Microbial Strains Mycobacterium tuberculosis Erdman Strain ATCC Cat#35801 BL21 (DE3) competent E. Coli New England BioLabs Cat#C2527I Recombinant DNA Plasmid pET-28a(+)-MtbRv271 GenScript N/A Plasmid pET-24d-MtbFDTS GenScript N/A Plasmid pET-41a(+)-MtbDHFR GenScript N/A Plasmid pET-41a(+)-HuDHFR GenScript N/A Deposited Data Macromolecular Crystallography Structure of Mtb DHFR bound to UCP1172 This study PDB ID: 6DDP Structure of Mtb DHFR bound to UCP1175 This study PDB ID: 6DDS Structure of Mtb DHFR bound to PAS-M This study PDB ID: 6DDW Structure of Hu DHFR bound to UCP1172 This study PDB ID: 6DE4 Structure of Mtb Rv2671 bound to UCP1063 This study PDB ID: 6DE5 Genome Sequences (BioProject ID: PRJNA523029) Whole Genome Sequence of Wild-type Mtb This paper BioSample accession: SAMN10962817 Whole Genome Sequence of PAS-R folC (E153G) mutant This paper BioSample accession: SAMN10962818 Whole Genome Sequence of PAS-R folC (I43T) mutant This paper BioSample accession: SAMN10962820 Whole Genome Sequence of PAS-R thyX mutant This paper BioSample accession: SAMN10962819 Open in a separate window KEY RESOURCE TABLE

Techniques:

Journal: Cell chemical biology

Article Title: Drugging the Folate Pathway in Mycobacterium Tuberculosis : The Role of Multi-Targeting Agents

doi: 10.1016/j.chembiol.2019.02.013

Figure Lengend Snippet: Left: Autoradiograph of TLC analysis of lipids extracted from 14C acetate labeled Mtb Erdman cells treated with UPC1172, PAS or DMSO as a control (CTR); Autoradiograph of standard (middle) and argentation (right) TLC analysis of methyl esters of fatty and mycolic acids isolated from 14C acetate labeled Mtb Erdman cells treated with UPC1172, PAS, or DMSO as a control (CTR)

Article Snippet: The accession numbers are provided in . table ft1 table-wrap mode="anchored" t5 caption a7 Reagents or Resources Source Identifier Chemicals Beta-nicotinamide adenine dinucleotide phosphate (NADPH) Alfa-Aesar Cat#J60387 Dihydrofolic acid (DHF) Sigma-Aldrich Cat#D7006 Tetrahydrofolic acid (THF) Sigma-Aldrich Cat#T3125 Methotrexate Sigma-Aldrich Cat#A6770 Isoniazid Sigma-Aldrich Cat#I3377 Flavin adenine dinucleotide disodium salt hydrate (FAD) Sigma-Aldrich Cat#F6625 2’-deoxyuridine-5’-monophosphate disodium salt (dUMP) Alfa-Aesar Cat#J64627 Thymidine 5’-monophosphate disodium salt (dTMP) Sigma-Aldrich Cat#T7004 5,10-Methylenetetrahydrofolate (mTHF) Toronto Research Chemicals Cat#F680350 Isopropyl-1-thio-b-D-galacto-pyranoside (IPTG) Thermo Scientific Cat#R0392 para -Aminosalicylic acid Sigma-Aldrich Cat#A79604 Nextera XT DNA library preparation kit Illumina Cat#FC-131 AMPure XP beads Beckman-Coulter Cat#A63880 Microbial Strains Mycobacterium tuberculosis Erdman Strain ATCC Cat#35801 BL21 (DE3) competent E. Coli New England BioLabs Cat#C2527I Recombinant DNA Plasmid pET-28a(+)-MtbRv271 GenScript N/A Plasmid pET-24d-MtbFDTS GenScript N/A Plasmid pET-41a(+)-MtbDHFR GenScript N/A Plasmid pET-41a(+)-HuDHFR GenScript N/A Deposited Data Macromolecular Crystallography Structure of Mtb DHFR bound to UCP1172 This study PDB ID: 6DDP Structure of Mtb DHFR bound to UCP1175 This study PDB ID: 6DDS Structure of Mtb DHFR bound to PAS-M This study PDB ID: 6DDW Structure of Hu DHFR bound to UCP1172 This study PDB ID: 6DE4 Structure of Mtb Rv2671 bound to UCP1063 This study PDB ID: 6DE5 Genome Sequences (BioProject ID: PRJNA523029) Whole Genome Sequence of Wild-type Mtb This paper BioSample accession: SAMN10962817 Whole Genome Sequence of PAS-R folC (E153G) mutant This paper BioSample accession: SAMN10962818 Whole Genome Sequence of PAS-R folC (I43T) mutant This paper BioSample accession: SAMN10962820 Whole Genome Sequence of PAS-R thyX mutant This paper BioSample accession: SAMN10962819 Open in a separate window KEY RESOURCE TABLE

Techniques: Autoradiography, Labeling, Control, Isolation

Journal: Cell chemical biology

Article Title: Drugging the Folate Pathway in Mycobacterium Tuberculosis : The Role of Multi-Targeting Agents

doi: 10.1016/j.chembiol.2019.02.013

Figure Lengend Snippet: KEY RESOURCE TABLE

Article Snippet: The accession numbers are provided in . table ft1 table-wrap mode="anchored" t5 caption a7 Reagents or Resources Source Identifier Chemicals Beta-nicotinamide adenine dinucleotide phosphate (NADPH) Alfa-Aesar Cat#J60387 Dihydrofolic acid (DHF) Sigma-Aldrich Cat#D7006 Tetrahydrofolic acid (THF) Sigma-Aldrich Cat#T3125 Methotrexate Sigma-Aldrich Cat#A6770 Isoniazid Sigma-Aldrich Cat#I3377 Flavin adenine dinucleotide disodium salt hydrate (FAD) Sigma-Aldrich Cat#F6625 2’-deoxyuridine-5’-monophosphate disodium salt (dUMP) Alfa-Aesar Cat#J64627 Thymidine 5’-monophosphate disodium salt (dTMP) Sigma-Aldrich Cat#T7004 5,10-Methylenetetrahydrofolate (mTHF) Toronto Research Chemicals Cat#F680350 Isopropyl-1-thio-b-D-galacto-pyranoside (IPTG) Thermo Scientific Cat#R0392 para -Aminosalicylic acid Sigma-Aldrich Cat#A79604 Nextera XT DNA library preparation kit Illumina Cat#FC-131 AMPure XP beads Beckman-Coulter Cat#A63880 Microbial Strains Mycobacterium tuberculosis Erdman Strain ATCC Cat#35801 BL21 (DE3) competent E. Coli New England BioLabs Cat#C2527I Recombinant DNA Plasmid pET-28a(+)-MtbRv271 GenScript N/A Plasmid pET-24d-MtbFDTS GenScript N/A Plasmid pET-41a(+)-MtbDHFR GenScript N/A Plasmid pET-41a(+)-HuDHFR GenScript N/A Deposited Data Macromolecular Crystallography Structure of Mtb DHFR bound to UCP1172 This study PDB ID: 6DDP Structure of Mtb DHFR bound to UCP1175 This study PDB ID: 6DDS Structure of Mtb DHFR bound to PAS-M This study PDB ID: 6DDW Structure of Hu DHFR bound to UCP1172 This study PDB ID: 6DE4 Structure of Mtb Rv2671 bound to UCP1063 This study PDB ID: 6DE5 Genome Sequences (BioProject ID: PRJNA523029) Whole Genome Sequence of Wild-type Mtb This paper BioSample accession: SAMN10962817 Whole Genome Sequence of PAS-R folC (E153G) mutant This paper BioSample accession: SAMN10962818 Whole Genome Sequence of PAS-R folC (I43T) mutant This paper BioSample accession: SAMN10962820 Whole Genome Sequence of PAS-R thyX mutant This paper BioSample accession: SAMN10962819 Open in a separate window KEY RESOURCE TABLE

Techniques: DNA Library Preparation, Recombinant, Plasmid Preparation, Sequencing, Mutagenesis